Abstract: The chemical and mineral characteristics of low grade vanadium titano-magnetite in Subei of Gansu Province had been studied by the ways such as X-fluorescence spectrophotometer and chemical multielement analysis and microscopic method and MLA mineral automatic analysis. The research results showed the content of metallic minerals was much less than non-metallic minerals and the percentage of metallic minerals in the ore was 9.06% and a wide range of non-metallic minerals in the ore widely existed. The content of magnetic iron minerals and ilmenite was low and the granularities of magnetite mineral grain was far thinner than ilmenite and the co-existence relation between magnetite and ilmenite was very close-knit. The grain dianeters of main non-metallic minerals were thick such as olivine, hornblende, pyroxene and feldspar. The important reference frame had been provided in this research which could be used to evaluate the vanadium titano-magnetite deposit.