解钾JX-10菌的筛选及解钾工艺

薛永萍; 肖春桥; 张琰图; 池汝安

doi:10.3969/j.issn.1000-6532.2023.02.004

解钾JX-10菌的筛选及解钾工艺

doi: 10.3969/j.issn.1000-6532.2023.02.004

薛永萍^{1, 2,},
肖春桥³,
张琰图⁴,
池汝安^1, ,

1.
武汉工程大学兴发矿业学院，湖北　武汉　430073
2.
武汉工程大学邮电与信息工程学院，湖北　武汉　430073
3.
武汉工程大学环境与生物工程学院，湖北　武汉　430073
4.
延安大学化学与化工学院，陕西　延安　716000

基金项目: 国家自然科学基金（51674178）；国家重点研发计划（2018YFC1801800）。

详细信息

作者简介:
薛永萍（1979-），女，博士，副教授，主要从事微生物冶金等研究。

通讯作者:
池汝安（1959-），男，博士，教授，主要从事稀土矿物加工及分离等研究。

中图分类号: TD981
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- 被引次数: 0
出版历程
- 收稿日期: 2021-02-01
- 修回日期: 2021-05-06

Screening of Potassium Dissolving JX-10 Bacteria and Research on the Optimization of Potassium-dissolving

1.
School of Xingfa Mining Engineering, Wuhan Institute of Technology, Wuhan, Hubei, China
2.
College of Post and Telecommunication of Wuhan Institute of Technology, Wuhan, Hubei, China
3.
School of Environmental and Biological Engineering, Wuhan Institute of Technology, Wuhan, Hubei, China
4.
College of Chemistry & Chemical Engineering, Yan′an University, Yan′an, Shaanxi, China

摘要

摘要: 本文从一钾长石矿区土壤中分离、筛选得到了6株高效解钾细菌。其中命名为JX-10的菌株分离于矿区茼蒿根系土壤，经基因测序鉴定为Bacillus sp.，与KT981886菌相似度高到99.72%。同时就培养时间、温度及转速等解钾工艺条件进行了探讨。实验结果表明，JX-10菌株具有分解钾长石矿物的能力。其较佳解钾工艺条件为：培养温度28 ℃、时间10 d，转速160 r/min，培养基pH值为 5.0，钾长石浓度2 g/L，粒度0.03 mm，接种量25%，硫酸铵浓度0.2 g/L。在较佳工艺条件下，溶液中可溶性钾离子含量可达23.32 mg/L，浸出率为8.36%。
- 解钾菌 /
- 筛选 /
- 鉴定 /
- 工艺优化
Abstract: In this study, six strains with potassium-dissolving ability were screened from the soils collected from a K-feldspar mining area . One of them, which named JX-10 strain was identified as a kind of bacterium, by 16 S rRNA gene sequencing. Simultaneously, the optimization of potassium-dissolving were also discussed. The results show that, the JX-10 strain had an obvious dissolution effect on K-feldspar. The optimum conditions for the JX-10 strain to remove potassium from K-feldspar were as follows: cultured at 28 ℃ for 10 days, pH value of the culture medium of 5.0, 60 mL medium in a 250 mL conical flask, and 160 r/min shaking speed on a rotary shaker. The K-feldspar concentration and granularity, inoculation volume, ammonium sulfate dose were 2 g/L, 0.03 mm, 25%, 0.2 g/L, respectively. Under the above conditions, the highest the potassium content and corrosion efficiency reach to 23.32 mg/L and 8.36%, respectively.
- Potassium bacteria /
- Screening /
- Identified /
- Process optimization

HTML全文

图 1 JX-10菌系统发育进化

Figure 1. Phylogenetic tree of the JX-10 strain

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图 2 JX-10菌株分解钾矿红外图谱

Figure 2. Infrared spectrum of k-feldspar decomposed by JX-10 strain

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图 3 培养时间对溶液中可溶性钾离子含量的影响

Figure 3. Effect of different culture time on the soluble potassium content

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图 4 培养温度对溶液中可溶性钾离子含量的影响

Figure 4. Effect of the cultivation temperature on the soluble potassium content

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图 5 培养转速对溶液中可溶性钾离子含量的影响

Figure 5. Effect of shaking speed during cultivation on the soluble potassium content

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图 6 培养基初始pH值对溶液中可溶性钾离子含量的影响

Figure 6. Effect of pH value of the culture medium on the soluble potassium content

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图 7 矿粉浓度对溶液中可溶性钾离子含量的影响

Figure 7. Effect of the K-feldspar concentration on corrosion efficiency

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图 8 矿粉粒度对溶液中可溶性钾离子含量的影响

Figure 8. Effect of K-feldspar granularity on the soluble potassium content

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图 9 接种量对溶液中可溶性钾离子含量的影响

Figure 9. Effect of the inoculation volume on the soluble potassium content

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图 10 硫酸铵浓度对溶液中可溶性钾离子含量的影响

Figure 10. Effect of the ammonium sulfate dose on the soluble potassium content

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