Abstract: ObjectiveTo make up for the deficiencies of anionic collectors such as sodium oleate in the reverse flotation of iron ores, Methodin this article, the combination of three non-polar oil co-collectors with different hydrocarbon chain lengths and the traditional anion collector sodium oleate was explored, and the pure mineral comparison test of sodium oleate, sodium oleate + diesel, sodium oleate + ethyl oleate, sodium oleate + trioleic glyceryl ester was carried out using quartz and hematite pure minerals as the research object. At the same time, the infrared spectroscopy was used to study the mechanism of action of sodium oleate + trioleic glycerol on the quartz surface. The proportioning experimental results showed that the flotation effect of sodium oleate + trioleic glyceride combined collector was relatively good at 4:1. ResultThe results of the artificial mixed ore test showed that the combination of sodium oleate + trioleate glyceride was able to obtain excellent indicators of concentrate grade of 64.13% and recovery rate of 91.50% at the optimal conditions of pH value 11.5, collector dosage of 120 mg/L, inhibitor dosage of 250 mg/L and activator dosage of 40 mg/L, and the dosage of pharmaceutical dosage was reduced by 52.00%. Conclusion The results of the infrared spectroscopy showed that triolein could promote the adsorption of sodium oleate on the surface of quartz, thereby improving the effect of hematite anti-flotation.